Could you please suggest me that how can I detach MCF10 A cells for passing?

Previously, I used 0.05% Trypsin-EDTA (used 1.5-2.5 ml for T75 flask after one PBS wash) to detach them, it took 20-30 min incubation at 37 and 5% CO2. And the cells took very long time (12-15...

01 January 2020 4,328 6 View