Shirin Mirlohi

4 Questions 26 Answers 0 Followers

Questions related from Shirin Mirlohi

Low OD of plasmid after isolation. Any idea?

I repeatedly get very low plasmid after isolation recently. I tried using 3 different kits but the problem persists. I usually put my cultures for growing over night and isolate the plasmid the...

12 December 2016 3,394 5 View

Difference between biological replicates in real-time PCR.

I work on microRNAs for my master thesis. Recently I've faced a problem in real-time PCR. I have 2 biological repeats and 3 technical repeats for each sample to detect the microRNAs expression...

05 May 2013 380 6 View

Database for analyzing the UTR region of viruses?

Does anyone know if there are any databases for analysing the UTR region of the flu virus? I don't know if I can use human databases for predicting the regulatory elements located on the UTR...

05 May 2013 5,442 0 View

Is 5'-RACE PCR enough for miRNA target gene confirmation?

Hello everyone, I have a candidate gene which I think is the target of a specific microRNA I work on. In order to confirm that theory, I decided to run a RACE-PCR. But right now I'm wondering...

01 January 1970 8,404 8 View