6 Questions 7 Answers 0 Followers
Questions related from Shinjinee Sengupta
I am working with various cell lines and have transfected Luciferease firefly plasmid and Renilla control plasmid for quantification of the signal. However, I am not able to get any reading with...
11 November 2019 729 4 View
I am constructing a DNA stretch with many genes under a single promoter by overlap extension PCR. The genes are joined in such a way such that the stop codon of one genes is followed by the start...
05 May 2018 8,002 3 View
I am transforming a local isolate of cyanobacteria with a construct as follows: Promoter-eyfp-terminator. After transforming the construct I am only getting the eyfp band and my antibiotic,...
12 December 2017 4,095 1 View
I am facing a segregation issue after transformation in cyanobacteria. The construct is not getting 100% segregated even after slowly increasing the antibiotic concentration. At high antibiotic...
12 December 2017 915 3 View
I was doing pathway engineering which involved joining of 6 DNA fragments. I have joined fragments 1 and 2 and named it as A. I have joined fragment 3 to 6 and names it as B. Now when I am joining...
11 November 2017 1,068 5 View
I am transforming cyanobacteria with a vector containing antibiotic gene as well as my construct. However, after transformation the strain is growing in very high concentration of antibiotic...
09 September 2017 7,370 8 View
