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I am working with various cell lines and have transfected Luciferease firefly plasmid and Renilla control plasmid for quantification of the signal. However, I am not able to get any reading with...
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I am constructing a DNA stretch with many genes under a single promoter by overlap extension PCR. The genes are joined in such a way such that the stop codon of one genes is followed by the start...
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I am transforming a local isolate of cyanobacteria with a construct as follows: Promoter-eyfp-terminator. After transforming the construct I am only getting the eyfp band and my antibiotic,...
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I am facing a segregation issue after transformation in cyanobacteria. The construct is not getting 100% segregated even after slowly increasing the antibiotic concentration. At high antibiotic...
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I was doing pathway engineering which involved joining of 6 DNA fragments. I have joined fragments 1 and 2 and named it as A. I have joined fragment 3 to 6 and names it as B. Now when I am joining...
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I am transforming cyanobacteria with a vector containing antibiotic gene as well as my construct. However, after transformation the strain is growing in very high concentration of antibiotic...
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