Shimaa A. Abdellatef

3 Questions 6 Answers 0 Followers

Questions related from Shimaa A. Abdellatef

How I make control for co-immunoprecipitation experiments?

I conducted a co-immunoprecipitation using protein X antibody to determine if protein X is attached to protein Y. However, the results of my experiments were unusual. To ensure that the procedure...

13 November 2023 9,775 1 View

Do u have any tips for blotting of integrin b1?

I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...

01 December 2020 4,318 3 View

How I choose representative values to get the variance in motion when I calculate the trap stiffness of optical tweezer?

I would use the variance in the Brownian motion using equipartition theorem to calculate the trap stiffness, but I could not understand how I choose the points that is representative to be used...

20 April 2018 5,502 2 View