Shimaa A. Abdellatef

4 Questions 8 Answers 0 Followers

Questions related from Shimaa A. Abdellatef

How I make control for co-immunoprecipitation experiments?

I conducted a co-immunoprecipitation using protein X antibody to determine if protein X is attached to protein Y. However, the results of my experiments were unusual. To ensure that the procedure...

13 November 2023 9,824 1 View

Do u have any tips for blotting of integrin b1?

I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...

01 December 2020 4,379 3 View

How I choose representative values to get the variance in motion when I calculate the trap stiffness of optical tweezer?

I would use the variance in the Brownian motion using equipartition theorem to calculate the trap stiffness, but I could not understand how I choose the points that is representative to be used...

20 April 2018 5,543 2 View

How I can make my siRNA solution more concentrated after I already added RNase free water?

I have siRNA-FAM labelled as -ve control. and I diluted it as company recommended to be 50uM (I use 1 ul equivalent to 50 picomole) ,and I want to make it 250 uM (so 1 ul would be equivalent to...

11 May 2016 2,776 6 View