Shikha Jindal

4 Questions 15 Answers 0 Followers

Questions related from Shikha Jindal

Does anyone works on 13C Labelled metabolomics by GC/LC-MS?What is the ratio of unlabelled: labelled substrate?

I need to know the protocol for the metabolic profiling through GC-MS/LC-MS for the 13C oleic acid.

13 August 2018 9,415 7 View

How long can a stab of E.coli survives at room temperature without mutations?

Due to some issue, i received the stab culture of e.coli after two weeks. I am worried about the culture.

13 August 2018 9,632 4 View

What are the external metabolites while degrading fatty acids as a substrate? How to quantify oleate in HP-5ms column for GC-MS?

I want to make a standard plot for the oleate by using different concentrations without using derivitization. I want to calculate oleate `s uptake rate in e.coli . Derivitization would be tough...

19 December 2017 4,728 15 View

Tergitol NP-40 or tween should be used as a surfactant for solubilising oleate in M9 media?

Surfactants like tergitolNP-40 and tween are frequently reported in the paper but my concern is to increase growth rate and to decrease foaming.

15 June 2017 3,138 1 View