Shi Li

6 Questions 6 Answers 0 Followers

Questions related from Shi Li

Recommendations for wesbites/programs for metabolic pathway analysis for metagenomics data?

I have microbiome sequencing data and I have analysed it to determine which taxa are up or down-regulated with my treatment, however, this doesn't really tell me much about the metabolic changes....

26 August 2021 7,962 6 View

Should you only use significant proteins when performing pathway analysis or your whole dataset (after filtering etc)?

When performing pathway analysis, should you only include proteins that were significantly changed (after performing t-tests, ANOVA etc), or should you include all your proteins from the dataset...

24 May 2021 1,458 3 View

How do you impute data that is not normally distributed?

I have proteomics data that have missing values. What is the best way to impute the data if my data is not normally distributed? I have 4 treatment groups, and I have scenarios where one...

24 May 2021 232 1 View

Does anyone have any suggestions for lipopolysaccharide kits that can be used for rat serum and/or rat tissues?

I want to measure the levels of lipopolysaccharide (LPS) in rat serum and if possible, rat brain tissue samples. There are a lot of kits out there that can measure LPS in serum but I wasn't sure...

11 October 2020 257 0 View

When performing mutliple behavioural tests over a period of time, should you habituate the animals before every test or just once at the beginning?

I am doing a baseline + 2 rounds of behavioural testing (NOR + NLR) - so total 3 rounds of testing, all 1 week apart in male SD rats. Is it necessary to habituate them before every test, or is...

18 June 2020 9,180 5 View

Does anyone have a protocol for tissue preparation and staining of endothelial cells in rat brain sections?

I'm having some trouble trying to stain for endothelial cell in rat brain tissues. My positive control works for CD31, but my samples are not coming up. I'm wondering if it is my tissue...

26 July 2017 4,464 3 View