Can liposomes be formed using DPPC lipid only in acetate buffer pH 5.3?

I made liposomes using Ethanol injection method, I try DPPC (2.5, 3.4, 5 mM) in acetate buffer. I fixed  the volume ratio of Etoh : buffer (pH 5.3) to 1:20 but i think No Liposome were formed...

10 October 2015 1,041 7 View