3 Questions 9 Answers 0 Followers
Questions related from Sherif Arafa
I have a protein crustal that grow under conditions of 25 % PEG 4000 , 0.1 M sofium acetate pH 4.9 and ammonium sulfate ..but the resolution was very bad around 8 .. the cryo protectant contains...
05 May 2019 8,458 1 View
Based on Size exclusion chromatography, my protein is a dimer. However, after solving the crystal structure, there was only one monomer. Is there a specific reason for that.
01 January 2019 7,748 6 View
I am trying to purify a certain protien i transformed into BL21 competent cells and do the purification steps but after the Ni affinity column and trying to run on ion exchange there was no peaks...
04 April 2018 1,884 4 View