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I did the PFGE of Bacillus cereus many times, but there were no bands on the gel. The protocol reference is https://pubmed.ncbi.nlm.nih.gov/25862049/ What's the problem with the experiment?
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50 ng/mL PMA for 24 hours to differentiate THP-1 cells to macrophages. Treat with LPS 100 ng/ml, 24hr and collect the supernatant to do ELISA. IL-1b does not express. Do you have any ideas?
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