Sheila Boreiko Sanchez

3 Questions 1 Answers 0 Followers

Questions related from Sheila Boreiko Sanchez

What percentage of glycerol ethoxylate is required to consider the condition of cryoprotectant crystallization?

I obtained protein crystals using the MIDAS Kit under the following condition: 30% v/v glycerol ethoxylate, 0.1 M Mes pH 6.5 and 0.2 M ammonium acetate. I would like to know if this condition is...

22 November 2019 6,587 3 View

How could I get more protein in the soluble fraction?

I need to express a Recombinant protein, I did the first test at 37 ° C with 0.5 mM IPTG for 6 hours of induction. It expressed a lot, but all in the insoluble fraction (inclusion bodies). I did a...

27 October 2019 8,126 3 View

How to choose between vectors pET15b (T7 promoter / lac operator) or pET14b (T7 promoter)?

As I understand, the only difference between them is that pET14b contains the T7 promoter whereas in pET15b T7 promoter plus lac operator. I read that T7/lac increases the chance of expression of...

27 August 2019 686 1 View