Sheikh Gulfam

4 Questions 26 Answers 0 Followers

Questions related from Sheikh Gulfam

Why the actin (250bp) shows a good melting peak but shows smear on Gel(2%)?

Hello everybody,I am optimizing transporter protein primers of coleus with actin(250bp). I used normal qPCR conditions for the optimization with annealing at 55oC and cycles 55. Most of primers...

01 January 2017 7,232 16 View

Which is the best Reference/ control gene for expression profiling in case of Plants?

Hello everybody , Can anybody guide me about the best gene for plants as control for expression profiling(qPCR). i m working on Coleus forskohlii ABC transporter proteins

01 January 2017 9,770 7 View

Why my PCR product sometimes shows smear and sometimes bigger size than should be ?

My problem is related with ITS primer .Previously i had no problem but from fews days bands on 1% agarose gel sometimes show smears, sometimes multiple bands and sometimes increase in amplicon...

11 November 2016 9,452 7 View

What is the precise role of ethanol in DNA extraction ?

It is well known that Ethanol has a lower dielectric constant than water, making it to promote ionic bond formations between the Na+ (from the salt) and the PO3- (from the DNA backbone),...

11 November 2016 1,202 10 View