I am trying to study whole cell ROS using H2DCFDA. But why does my TNF alpha treated cells show less fluorescence intensity than my control cells?

I am working with human synovial sarcoma cells and use the microplate reader protocol for detecting whole-cell ROS. I use phenol red free DMEM- low glucose +10%FBS to grow the cells and add 9uM of...

12 October 2019 2,792 7 View