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I am working with the E. coli strain BAS901, which has a mutation in lptD rendering the cells hyperpermeable via defective LPS assembly. I need to introduce a plasmid into this strain....
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I am interested in detecting inhibition of lipoprotein transport to the outer membrane of E. coli. In several papers (Cock, 2005, JBC), I have noticed that authors created spheroplasts,...
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