Shaun Wood

3 Questions 10 Answers 0 Followers

Questions related from Shaun Wood

What is the optimal distance between the promoter and start codon?

Hi there, I'm trying to clone a promoter into a plasmid vector and I was wondering if there is an optimal distance between the promoter and the AUG at the start of my transgene? Thanks in advance...

07 July 2014 2,266 5 View

KpnI and BglII restriction digestion. Can they be done in the same reaction?

I'm looking to perform a restriction digest using KpnI and BglII however all the suppliers I look at (Promega, NEB, etc) all have the two enzymes operating in different buffers. Does anyone know...

03 March 2014 613 6 View

Anyone got any suggestions for isolating genomic DNA from HEK 293 cells without using a commercial kit?

Hi all, I'm trying to isolate some gDNA from HEK 293 cells for use in PCR reactions and was wondering if anyone could suggest a method for lysing the cells that I could use without buying in a...

11 November 2013 6,107 4 View