Shaun Wood

3 Questions 6 Answers 0 Followers

Questions related from Shaun Wood

What is the optimal distance between the promoter and start codon?

Hi there, I'm trying to clone a promoter into a plasmid vector and I was wondering if there is an optimal distance between the promoter and the AUG at the start of my transgene? Thanks in advance...

28 July 2014 361 4 View

Can anyone help with passaging of ARPE19 Cells?

I'm working with some ARPE19 cells but I'm having some issues when it comes to passaging them. They seem unwilling to detach from the culture flask when incubated with 1x Trypsin at 37 degrees C...

20 May 2014 9,421 3 View

Anyone got any suggestions for isolating genomic DNA from HEK 293 cells without using a commercial kit?

Hi all, I'm trying to isolate some gDNA from HEK 293 cells for use in PCR reactions and was wondering if anyone could suggest a method for lysing the cells that I could use without buying in a...

21 November 2013 7,028 3 View