Shatakshi Shukla

2 Questions 5 Answers 0 Followers

Questions related from Shatakshi Shukla

How to inhibit Non specific binding in RNA-IP pull down ?

Our group is trying to pull down AGO-FLAG protein to look for miRNAs interacting with AGO2, however we are getting expression at the same Ct for non-specific miRNAs in the negative control group...

12 February 2022 7,621 1 View

The cell lines have become sensitive to the treatment conditions. This has never happened in the previously done experiments. What is the reason ?

I am working on neuronal cell line SH-SY5Y and glial cell line U-87 MG using 6-OHDA and rotenone as PD stress inducing agents. I am transfecting the cells followed by the treatment. After 24hrs of...

22 November 2021 8,899 5 View