Shasha Alice Cheng

2 Questions 1 Answers 0 Followers

Questions related from Shasha Alice Cheng

Why different size of Fc-fusion protein after purified with protein A beads, and run Coomassie Brilliant Blue gel, I got the same bands?

I have different sizes of Fc fusion plasmids, I transfected with 293T cells and purified with protein A beads. After that, I run the protein gel and found that they showed the same bands. But If...

19 June 2023 7,718 3 View

It is beetter to use primary cell lines or Immortalized cell lines in CRISPR–Cas9 base edit?

I plan to do the CRISPR-cas9 base edit in mouse cells, it is better to use the primary cells or immortalized cell lines? I have read some papers, some used the primary cell lines.

29 December 2022 420 1 View