Why different size of Fc-fusion protein after purified with protein A beads, and run Coomassie Brilliant Blue gel, I got the same bands?

I have different sizes of Fc fusion plasmids, I transfected with 293T cells and purified with protein A beads. After that, I run the protein gel and found that they showed the same bands. But If...

19 June 2023 7,708 3 View