Sharmila Narayanan

2 Questions 4 Answers 0 Followers

Questions related from Sharmila Narayanan

How to use azocoll as a substrate for determining recombinant serine protease?

I am working on recombinant subtilisin-like serine protease. As I have to analyze whether my recombinant protein of interest is active or not, I am using azocoll as substrate. I have used...

04 April 2014 7,629 3 View

Cell lysis buffer composition for his tagged protein purification in soluble fraction?

The protein I am working on is quite large (Approx 80kDa). I used the cell lysis buffer (PBS, PMSF and Triton X- 100) and finally I found my protein is present in the pellet. I am confused about...

06 June 2013 10,006 8 View