Sharmila Mujawar

2 Questions 1 Answers 0 Followers

Questions related from Sharmila Mujawar

How can I prevent degradation of protein?

I am working on Mus musculus protein of 85 KD which expresses very well but I am getting degradation band so for confirmation I did Mass Spec and western blot so its degrading from the C terminus...

11 November 2015 3,350 10 View

How can I improve protein crystals?

Since last few months I am trying to improve my 40kd protein crystals for which I am getting either needles or multinucleation. I have tried various conditions with varying pH, Temperature,...

11 November 2015 5,795 4 View