Sharmila Narayanan

3 Questions 7 Answers 0 Followers

Questions related from Sharmila Narayanan

Can you help me with transient transfection?

We have cloned our gene in mammalian expression vector pEGFN1 vector by introducing a stop codon between the gene of interest and EGFP sequence so that no GFP is expressed. While performing...

09 April 2015 1,130 6 View

How to use azocoll as a substrate for determining recombinant serine protease?

I am working on recombinant subtilisin-like serine protease. As I have to analyze whether my recombinant protein of interest is active or not, I am using azocoll as substrate. I have used...

05 April 2014 5,431 2 View

Cell lysis buffer composition for his tagged protein purification in soluble fraction?

The protein I am working on is quite large (Approx 80kDa). I used the cell lysis buffer (PBS, PMSF and Triton X- 100) and finally I found my protein is present in the pellet. I am confused about...

05 June 2013 1,567 7 View