Why BxPC3 pancreatic cancer cells die due to Akt plasmid transfection with Lipofectamine 2000? There is approxiamtely 60%-70% cell death?

I transfected BxPC3 cells with 5µg Akt plasmid using Lipofectamine 2000. I also had a control where I added only Lipofectamine 2000. Surprisingly, I observed 60-70% cell death in the wells...

15 August 2018 2,731 3 View