Sharat Varma

3 Questions 3 Answers 0 Followers

Questions related from Sharat Varma

Is there a possibility of IL10R involvement in the development of resistant UC-overlap?

There is a 5 year old child who developed UC at 2 years and subsequently sclerosing cholangitis. Steroids / Immuran and Ramicade x 1 year are unable to induce remission. Is there a possibility of...

23 October 2014 6,019 1 View

Th17 staining in liver tissue?

Is anyone doing T17/Treg staining on liver biopsy specimens (paraffin fixed)? I am getting a huge background. Can I use ImageJ to quantify the degree of positivity I get, such that I could...

23 October 2014 9,620 2 View

How can I stimulate Th17 in a healthy donor?

I am trying to stimulate the PBMC from a healthy donor but its not happening. Do the healthy PBMCs get stimulated? I used PMA/Iono/LPS and IL23 overnight. Are there any tried and tested protocols...

25 June 2014 7,910 5 View