Sharad Kholia

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Questions related from Sharad Kholia

When we initialize DC differentiation using CD14+ monocytes some of the monocytes die. Do these dead cells have an effect on the DC differentiation?

I am differentiating PBMCs to iDC and currently leave the monocytes for 6 days with the cytokines, however after day 2 of 6 I notice dead cells (some monocytes and some DC). Do these cells have an...

24 March 2014 9,251 2 View

What are the best markers for immature Dendritic cells derived from CD14+ PBMC?

I am currently differentiating PBMC to iDC using 800iu/ml GMCSF and 500iu/ml IL4 for 5 days with change of media on day three by substituting half of the media with fresh media containing...

18 March 2014 3,721 16 View