When we initialize DC differentiation using CD14+ monocytes some of the monocytes die. Do these dead cells have an effect on the DC differentiation?

I am differentiating PBMCs to iDC and currently leave the monocytes for 6 days with the cytokines, however after day 2 of 6 I notice dead cells (some monocytes and some DC). Do these cells have an...

24 March 2014 9,233 2 View