In mitochondria copy number multiplex PCR, do you average Cts or final copy number for quadruplicates?

We have quadruplicates for mitochondria copy number multiplex PCR. ND1 representing the mitochondrial DNA and RNAse P being our reference. Equation for mitoCN= (2^(ND1 Ct-RNAseP Ct)) *...

02 April 2020 3,938 0 View