Shani Dror

6 Questions 7 Answers 0 Followers

Questions related from Shani Dror

How to measure rRNA amount?

Hi all, I try to figure out weather there is a change in my rRNA following treatment. when I treat the cells (mouse cells) and isolated RNA, I see a major difference in RNA concentration, without...

09 September 2018 4,031 7 View

Protocol for western for secreted proteins?

I collected CM from tissue (serum free FBS), so I have very high concetration of protein, no need to concentrate. can I just add sample buffer to the media, boil and do western? or I am missing...

10 October 2017 3,223 6 View

What is a good marker for breast cancer?

Hi, I want to check whether my breast cancer cell (E0771) metastasis to the lung using RT-PCR. which marker will be a good one for that?

04 April 2017 3,600 2 View

Anyone knows protocol for viva spin tubes?

Hi all, I want to do western blot for one of my secretome proteins. I thought to use viva spin in order to concentrate the medium.How should I proceed? can I add lysis buffer? how much?or should I...

07 July 2016 7,982 3 View

Could anyone suggest a dye used in flow cytometry that can stain specifically to RNA but not DNA?

I want to show RNA delivery between cells using stained RNA.

09 September 2015 7,974 3 View

Can anyone suggest a fibroblast marker?

I want to stain human paraffin section of melanoma. I need marker that stain only the fibroblast and not the melanoma. for example, vimentin stain some of the melanoma so its not good for me. Does...

06 June 2014 7,575 1 View