Shang-Te Danny Hsu

4 Questions 6 Answers 0 Followers

Questions related from Shang-Te Danny Hsu

Want to be a research assistant for membrane protein analysis?

Background requirements BA or MA in Life Sciences-related topics Versed in molecular biology, particularly in cloning for bacterial and higher organism expression systems Experienced in...

11 November 2015 3,444 0 View

Setting up MD simulations for glycans in Gromacs?

I'd like to run MD simulations of lectins in complex with glycans such as lactose using Gromacs. What is the best way to define the parameter and topology for lactose that are compatible with...

09 September 2015 7,083 5 View

Are there tricks to improve the efficiency of on-column digestion by TEV protease?

We are trying to remove a His-tagged fusion tag from the target protein by on-column TEV protease digestion during Ni-NTA purification, but the efficiency was quite low compared to the digestion...

09 September 2015 9,698 8 View

Why did FITC labelling fail at the end step of solid phase peptide synthesis?

We were synthesizing a short peptide (12 residues long) with FITC labelled at the N-terminus by a standard peptide synthesizer. Everything went well until the last step of FITC incorporation. Our...

09 September 2015 5,646 12 View