Shane Collett

2 Questions 3 Answers 0 Followers

Questions related from Shane Collett

How to predict / discover active site locations in proteins?

Hi all, I have a number of proteins mainly from specific protozoans, Dictyostelium discoideum & humans. I need to find the active sites of each. I've used phyre2 to generate the structures...

12 March 2021 6,377 3 View

Does GFP tagged organism "lose" fluorescence over time / each time when excited?

I'm working with the the organism Dictyostelium discoideum - specifically an AX4 strain which is GFP tagged. When I added a culture into a flat bottom 96 well plate and take a reading (485 to 535:...

19 August 2019 606 6 View