Do human neutrophils incubated with FURA 2 AM (1uM) at 37 C for 45 min. get activated and form clumps?

I am isolating human neutrophils for intracellular calcium estimation using FURA 2AM. I am able to get good number of resting cells but they are getting activated and form clumps only when...

02 December 2015 4,068 4 View