What could be the possible reasons for not getting a signal in x-ray film even if I can see the glow in my blot after adding luminol and H2O2?

When I am trying to develop my blot by ECL method, I am facing this problem. After all the antibody incubation and washes, when I am adding luminol/H2O2 on to the blot, I could literally see the...

01 January 1970 7,048 5 View