Shalini Roychoudhury

6 Questions 21 Answers 0 Followers

Questions related from Shalini Roychoudhury

Could anyone help with why the protein (Myc-DDK tagged) not expressing in HEK293T cells?

I am working on a protein which is encoded by 6.3kb of cDNA. The expected molecular weight of the protein is 236kDa. I have the commercially purchased cDNA clone of the same in a Myc-DDK backbone...

08 August 2014 6,322 6 View

Is there any reliable prediction software available for predicting intronic variations?

I want to predict the effect (if any) of intronic variations on the respective protein.

12 December 2013 6,803 1 View

Is there any software for predicting disulphide bonds in a protein?

I want to see the possible disulphide bond formations in a particular protein.

10 October 2013 9,354 18 View

What are the essential criteria for designing a probe for northern blot?

What should be the minimum probe length, the GC content and annealing temperature? Is a DNA or RNA probe more preferable? Is there any software to help design probes or can I just pick up a...

08 August 2013 6,431 7 View

I am using an older version of samtools (0.1.7a) for my project. Do I have any chance of missing out on data, SNPs or Indels, with the older version?

Difference between old and new version of samtools

10 October 2012 6,335 2 View

What are the best possible softwares for ORF prediction?

Prediction softwares especially with respect to structure and function

10 October 2012 4,154 9 View