Shalini Reddy

3 Questions 9 Answers 0 Followers

Questions related from Shalini Reddy

How to anneal RNA sequence to a complementary DNA sequence in a oligo ?

Can complementary RNA and DNA anneal or hybridize naturally at room temperature or should I denature and cool them as we do for annealing DNA oligos? Should I add reagents like DMSO or MgCl2 etc...

08 August 2019 5,449 4 View

What is the best way to purify the ear clip genomic DNA from TaqMan DNA extraction reagent?

I used TaqMan DNA extraction reagent (Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit) to prepare the mouse ear clip to screen for targeted gene by TaqMan qPCR and found some positive clones. But...

10 October 2018 1,771 4 View

Does CRISPR-Cas9 cause off-targets invivo in mice?

I have read couple of recent papers in biorxiv stating that CRISPR Cas9 gene editing does not cause significant off-target mutations invivo in mice. Is it that they could not see the off-targets...

03 March 2018 5,677 7 View