Shalini Roychoudhury

4 Questions 21 Answers 0 Followers

Questions related from Shalini Roychoudhury

Could anyone help with why the protein (Myc-DDK tagged) not expressing in HEK293T cells?

I am working on a protein which is encoded by 6.3kb of cDNA. The expected molecular weight of the protein is 236kDa. I have the commercially purchased cDNA clone of the same in a Myc-DDK backbone...

21 August 2014 2,700 5 View

Is there any software for predicting disulphide bonds in a protein?

I want to see the possible disulphide bond formations in a particular protein.

24 October 2013 2,730 18 View

What are the essential criteria for designing a probe for northern blot?

What should be the minimum probe length, the GC content and annealing temperature? Is a DNA or RNA probe more preferable? Is there any software to help design probes or can I just pick up a...

13 August 2013 8,241 6 View

What are the best possible softwares for ORF prediction?

Prediction softwares especially with respect to structure and function

02 October 2012 4,621 9 View