Shalini Reddy

3 Questions 6 Answers 0 Followers

Questions related from Shalini Reddy

What is the best way to purify the ear clip genomic DNA from TaqMan DNA extraction reagent?

I used TaqMan DNA extraction reagent (Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit) to prepare the mouse ear clip to screen for targeted gene by TaqMan qPCR and found some positive clones. But...

14 October 2018 3,540 3 View

Has anyone visualised ssDNA from dsDNA on a agarose gel?

I am separating ssDNA from dsDNA using streptavidin bead. I electrophoresed the sample after separation on agarose gel. I can see a band of 1.7kb (dsDNA) and a band of 1kb. I am not sure whether...

16 February 2017 7,271 6 View

How to seperate a ssDNA from dsDNA (~2kb) using Streptavidin Dyna Beads?

I'm using Streptavidin Dyna beads to seperate my ssDNA.I don't know how to purify the ssDNA from the NaOH solution. I need a pure ssDNA to inject in zygote. Any suggestions?

01 January 1970 6,681 3 View