Shalini Dubey

3 Questions 5 Answers 0 Followers

Questions related from Shalini Dubey

Global mean normalization for more than 5 miRNAs in more than 100 samples?

I am working with plasma miRNAs and struggling to obtain a stable endogenous control for the same. I am also using synthetic spike-ins during each technical step. I would be studying 8 microRNAs...

16 January 2020 3,321 0 View

Can anyone provide a reliable protocol with tips and tricks for miRNA isolation from human plasma using Trizol?

Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...

14 June 2019 2,305 9 View

Can anyone help me understanding why am I getting jagged amplification curve for my miRNAs in plasma sample?

I have used mircury exosome serum/plasma kit along with trizol for extraction of my miRNAs from human plasma. RNA concentration was 623.8ng on nanodrop with 260/280 ratio of 2.12. For RT-PCR and...

12 June 2019 3,776 4 View