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I have cultured HPDE cells twice. They were growing good enough and when confluent, I splitted them once with Trypsin and acutase separately but they are almost all dead and didnt attach to the...
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I need to harvest the secretome of mesenchymal stem cells. In order to do that, I used serum free medi and 75T culture flask but the concentration was low and not enough to run it on the array...
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Its easy somehow to culture MSCs in media containing serum, but how can I culture them in serum free media and keep them alive?
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