Shaikh Irfan

5 Questions 8 Answers 0 Followers

Questions related from Shaikh Irfan

What is the best way to concentrate Exosomes (100 -150 nm) size particles? Which MWCO Centricorn is suitable for further concentration?

This is after completion of TFF process. Answers are appreciated. Thanks.

12 August 2024 9,467 1 View

Does anybody share your experience about the usage of Nunc UpCell plates for human mesenchymal stem cells culture?

Your answers are appreciated. Thanks.

29 February 2024 8,570 0 View

Does anybody has done or aware of corelation of Luminiscence RLU units and titer of virus ?

I want to quantify pseudovirus carrying Luc gene. I have no idea how to corelate RLU units and pseudovirus titer? Please let me know if you are aware , Thank you in advance.

07 July 2020 9,524 0 View

Does anybody has an experience in Lentiviral transduction in Jurkat cells?

I have generated the lentivirus particles with my GOI. But did not get transduction efficiency in Jurkat cells post 72 hrs why? any suggestion ? Particulas: used 24 well plate : 50,000 cells with...

24 February 2016 6,489 3 View

1.Does anyone has experience with suspension cell line, making virus with lentiviral packaging mix (SBI)?

Hi everyone, I am using Jurkat cells for transduction with the lentivirus generated with SBI packaging mix. I used spinfection but did not work no gfp in gfp-control well. Second time I have added...

03 February 2016 8,664 4 View