Shaikh Irfan

4 Questions 7 Answers 0 Followers

Questions related from Shaikh Irfan

Does anybody share your experience about the usage of Nunc UpCell plates for human mesenchymal stem cells culture?

Your answers are appreciated. Thanks.

29 February 2024 8,564 0 View

Does anybody has done or aware of corelation of Luminiscence RLU units and titer of virus ?

I want to quantify pseudovirus carrying Luc gene. I have no idea how to corelate RLU units and pseudovirus titer? Please let me know if you are aware , Thank you in advance.

07 July 2020 9,522 0 View

Does anybody has an experience in Lentiviral transduction in Jurkat cells?

I have generated the lentivirus particles with my GOI. But did not get transduction efficiency in Jurkat cells post 72 hrs why? any suggestion ? Particulas: used 24 well plate : 50,000 cells with...

24 February 2016 6,486 3 View

1.Does anyone has experience with suspension cell line, making virus with lentiviral packaging mix (SBI)?

Hi everyone, I am using Jurkat cells for transduction with the lentivirus generated with SBI packaging mix. I used spinfection but did not work no gfp in gfp-control well. Second time I have added...

03 February 2016 8,661 4 View