Shahrzad Nouri

3 Questions 11 Answers 0 Followers

Questions related from Shahrzad Nouri

Why am I getting multiple bands in western blot (in Isolating adhesion complexes) ?

Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...

20 September 2020 681 7 View

Why I Can not see any organelles in TEM image of cells? Any tip for fixation step?

Hello TEM experts, I need to take a cross section TEM image of my cells. The first time I prepared the samples every thing looked normal. But, since then I am trying to repeat the experiment, the...

28 February 2020 6,593 6 View

What's the best protocol for diluting DMSO when thawing the primary human epithelial cells?

Hi, I have some problem with the viability of my primary epithelial cells after thawing. I have used two protocols (centrifuging or only diluting 10 times more and changing the media the day...

04 October 2019 8,070 6 View