Shahin Shabani

3 Questions 2 Answers 0 Followers

Questions related from Shahin Shabani

Why tissue section comes off the slide after antigen retrieval with EDTA pH9?

I have brain cryosections on gelatin coated slides. I keep them in -20 or -80. For IHC, I dry the sections for 30 min at room temperature, wash them for 15 min with PBS and start antigen retrieval...

16 January 2019 2,193 0 View

Nuclear extraction from brain tissue?

Hi, I'm looking for an inexpensive and efficient method to isolate nuclear fraction from brain tissue. There are kits and protocols available, but they are mostly for cells and tissues other than...

15 January 2019 523 2 View

What is the difference between Ca and Mg free HBSS and HBSS with MgCl2, MgSo4 and CaCl2 in tissue transferring/dissecting process?

I'm trying to start Organotypic slice culture of brain but there are many references using different components for the medium during dissection and afterward when culturing the slices. HBSS in...

22 October 2018 5,105 0 View