Shahid Rehan

3 Questions 9 Answers 0 Followers

Questions related from Shahid Rehan

Where to get 3D image for thesis/ journal cover page?

Hi, I want to make a 3D image as a graphical abstract of my research work. I saw some very cool 3D images in google. I was wondering where I can get these kind of images, Is there any online...

03 March 2017 1,676 1 View

How to estimate % protein activity from radioligand binding assay?

I did saturation binding assay with crude membrane and detergent purified protein. I have estimated the bound ligand (pmol/mg of protein). How I can estimate the % of protein that has retained the...

12 December 2016 7,670 7 View

Why a protein did not bind to metal free agarose while it binds to Ni, Zn and Co agarose? Protein has His tag.

I am purififying one membrane transporter protein from SF9 cells. I can see glycosylation of my protein on SDS-PAGE. I tested Ni-agarose, Co-agarose, Zn-agarose and metal free agarose matixes. I...

07 July 2013 8,142 4 View