Shahid Ali

4 Questions 4 Answers 0 Followers

Questions related from Shahid Ali

Can someone tell me , which vector and strain is best to express Thermomyces lanuginosus lipase in E. coli system?

Actually , i want to express Thermomyces lanuginosus lipase (TLL) in E coli system. so please help me and if somebody have protocol/procedure plz share with me. Thanks in advance!

13 May 2019 442 0 View

How can i place a protein in the center of prepared box with different solvents ?

ACtually ,i want to simulate a protein with different types of solvent present like Glycerol+urea+chloride. I created a box with these mix solvents. Now , i am facing the problem is that : how...

06 January 2019 8,860 5 View

How can i validate my method by statistically ?

I developed a method to predict substrate specificity ,but problem is that- method is just based on 6 enzymes(experimentally ) dataset.furthermore, i applied that method to unknown enzymes ,its...

29 August 2017 5,135 4 View

Can i use Rosetta Design for mutants design of enzyme ??

actually ,I am working on enzyme design by computational methods ,and i want improve enzyme activity and stability ,so can i used some other tools ?? thanks !!

20 February 2017 4,539 3 View