Shaheera Fatima

4 Questions 2 Answers 0 Followers

Questions related from Shaheera Fatima

Alternative options is available for Exosome depleted FBS?

When exosome-depleted FBS is not available. Also, ultracentrifugation is not available for manual preparation of Exosome depleted media. Any alternative options to avoid FBS exosomes?

08 August 2023 3,936 1 View

Why Plasmid DNA gel electrophoresis band is not shown?

If you see only two bands on your gel, how can you tell the plasmid's conformation? For example, it could be supercoiled and linear, or it could be linear and nicked circular or concatamer... This...

14 March 2023 4,530 9 View

How to make construct for plasmid that transfect HEK293T to produce targeted exosome by genetic engineering?

I am making a construct for a plasmid that transfect HEK293T. The thing I want to include in it is CD9 transmembrane protein, target peptide, and 6xHistag.I don't understand where 6xhistag is...

28 September 2022 3,325 1 View

Exosome isolation protocol

I need an exosome isolation protocol from HEK293 cells transfected with plasmid. I want to isolate exosomes from HEK 293 cells. Can someone share the protocol?

01 January 1970 6,028 1 View