Shaheera Fatima

8 Questions 3 Answers 0 Followers

Questions related from Shaheera Fatima

Which cheap FACS kit to use to isolate CD8 T cells?

Please recommend cheap and economical kits for CD8 T cells isolation using FACS, Chinese preferably.

11 October 2023 5,029 0 View

Alternative options is available for Exosome depleted FBS?

When exosome-depleted FBS is not available. Also, ultracentrifugation is not available for manual preparation of Exosome depleted media. Any alternative options to avoid FBS exosomes?

08 August 2023 3,947 1 View

I need good working protocol for exosome isolation by Polyethylene glycol methods?

I need good working protocol for exosome isolation from Hek293 cell line transfected with plasmid DNA by Polyethylene glycol methods. which peg is best for isolation peg4000, peg 6000, or peg...

08 August 2023 7,673 0 View

Is exosome pellet is visible after isolating from cell culture supernatant by Polythene isolation methods?

I am trying to make an exosome that expresses my gene of interest. I transfect the hek293 with a plasmid that has my gene of interest. After transfection, I isolate the exosomes from the cell...

31 March 2023 9,660 1 View

Why Plasmid DNA gel electrophoresis band is not shown?

If you see only two bands on your gel, how can you tell the plasmid's conformation? For example, it could be supercoiled and linear, or it could be linear and nicked circular or concatamer... This...

14 March 2023 4,538 9 View

Can using a same linker between 3 sequence elements cause a problem?

Hello everyone, I am currently working on designing a construct sequence for a peptide that is to be cloned in an expression vector. The construct I made has the following elements EcoR1...

22 November 2022 9,789 2 View

How to make construct for plasmid that transfect HEK293T to produce targeted exosome by genetic engineering?

I am making a construct for a plasmid that transfect HEK293T. The thing I want to include in it is CD9 transmembrane protein, target peptide, and 6xHistag.I don't understand where 6xhistag is...

28 September 2022 3,335 1 View

Exosome isolation protocol

I need an exosome isolation protocol from HEK293 cells transfected with plasmid. I want to isolate exosomes from HEK 293 cells. Can someone share the protocol?

01 January 1970 6,035 1 View