Shafieeh Mansoori

9 Questions 22 Answers 0 Followers

Questions related from Shafieeh Mansoori

What do you recommend to do for a successful ligation containing one vector and two inserts?

Hi everyone. I put a ligation reaction containing a 1 vector (~8 kb) and 2 inserts ( 434 and 537 bp) under bellow condition: vector: 3' xbaI....... AgeI 5' insert1: 3' xbaI ..... EcoRV 5' insert2:...

15 January 2024 5,587 2 View

How can I expand a transduced single jurkat cell ?

What additives can I add to the culture medium to improve the expansion of a Jurkat cell in a 96-wells plate? I have tried to isolate a transduced single jukat cell without a selection...

05 August 2021 5,532 7 View

Can I use an antibody with Elisa application for a lateral flow application?

hi everybody I want to develop a lateral flow test with the sandwich assay format. I searched for an antibody for the test line and found an antibody that has ELISA application (not lateral flow...

17 May 2021 2,833 11 View

How to choose a sample pad for a hCG lateral flow strip?

hei guys, I don't know how must I select a sample pad or conjugate pad for preparing a hCG Lateral flow assay. I know that what specifications are important but i do not know how can consider them...

16 April 2021 6,098 1 View

Can I RNase A treatment my extracted plamid?

I have extracted the plasmid with the Qiagen maxi Endofree kit. the result of gel electrophoresis for this sample showed a band of degraded RNAs. Now, I want to remove RNAs from the eluted plasmid...

26 January 2021 3,068 3 View

How to improve the lentivirus transduction efficiency in jurkat or parimary T cells?

Hi, I am transducing jurkat cells with lenti-virus but my efficiency rate is low... my transducing method is this: 1. I defreez viruses and put them 20 min in room temp. , 2. resuspending them in...

26 June 2020 9,188 3 View

Which cell lines are used for high-efficiency transfection?

Hi, I am trying to transfect my vector (about 10 kb) into the HEK293 T by reverse transfection method. I have a big problem with this cell line because it is semi suspend. When I want to change...

09 October 2019 4,875 3 View

How can I isolate tumor-reactive CD8+ T cells from a patient ?

Is there any standard protocol for isolating of tumor-reactive CD8+ T cells from a patient? I want to have a tumor antigen- specific T cell from a human, for example, isolating of CD4 plus and...

11 November 2018 2,949 6 View

How can Ì fix and permeable a cell line expressing GFP for proliferation assay using ki-67?

Hi guys I want to do proliferation assay with ki-67 for GFP+ jurkat cell line. The problem is GFP because when GFP+ jurkats are fixed with 4%formaldehid and then permeabled with 0.1% triton x100,...

01 January 1970 5,611 5 View