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Questions related from Shafieeh Mansoori
What additives can I add to the culture medium to improve the expansion of a Jurkat cell in a 96-wells plate? I have tried to isolate a transduced single jukat cell without a selection...
05 August 2021 5,525 7 View
hei guys, I don't know how must I select a sample pad or conjugate pad for preparing a hCG Lateral flow assay. I know that what specifications are important but i do not know how can consider them...
16 April 2021 6,091 1 View
I have extracted the plasmid with the Qiagen maxi Endofree kit. the result of gel electrophoresis for this sample showed a band of degraded RNAs. Now, I want to remove RNAs from the eluted plasmid...
26 January 2021 3,048 3 View
Hi, I am transducing jurkat cells with lenti-virus but my efficiency rate is low... my transducing method is this: 1. I defreez viruses and put them 20 min in room temp. , 2. resuspending them in...
26 June 2020 9,166 3 View
Hi, I am trying to transfect my vector (about 10 kb) into the HEK293 T by reverse transfection method. I have a big problem with this cell line because it is semi suspend. When I want to change...
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