Shadab Perveen

2 Questions 2 Answers 0 Followers

Questions related from Shadab Perveen

What would be the most suitable conditions when dealing with degenerate primers?

I am using degenerate primers for amplifying viral genome. At the beginning, the bands appeared at appropriate size but now nothing appears except for unspecific bands.

28 October 2012 6,520 1 View

I continuously get a smear or sometimes no band with amplified viral cDNA.

Does anyone have any suggestions?

30 April 2012 4,527 3 View