Shachinthaka Dissanayaka

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Questions related from Shachinthaka Dissanayaka

Is there any simple method to find the unit concentration of Taq polymerase ?

Standard method to define the unit concentration of Taq polymerase is based on incorporation of radioisotope-labeled dNTP, acid precipitation,and filtration. Since my lab does not have that...

08 April 2016 557 3 View

Why M-Mlv reverse transcriptase needs more units ( 200 units) for a reaction?

I am trying to find the optimum amount of M-Mlv rt enzyme (purified by myself) required for a RT-PCR . I compared the activity of my M-Mlv rt enzyme and other commercially available rt enzyme.I...

01 January 1970 8,217 5 View