WE tried to do ddPCR (in duplex) with two probes, one FAM and one VIC. The VIC signal is detected in channel 1 (which is FAM channel)! ANY IDEA WHY?

We are trying to adjust a ddPCR in duplex with two probe set: FAM and VIC. The 2D plot demonstrate 4 droplet fractions but all seems to be detected in channel 1 (FAM) even the VIC+ droplets. We...

27 February 2018 8,935 1 View