Seyed Nooreddin Faraji

5 Questions 10 Answers 0 Followers

Questions related from Seyed Nooreddin Faraji

Could anybody give me an idea about Dot blot problem?

Hi... I expressed a scFv antibody in HB2151 e.coli by TES (Tris-EDTA-sucrose) buffer and now like to confirm it by Dot blot, however, I failed to visualized it using DAB.I coat the scFv (with a C...

07 March 2017 8,306 11 View

Phage precipitation using PEG/NaCL. Mechanism of TBS buffer at the second phase of precipitation and that why we work on ice?

Hi I am working on filamentous phages. I wanna precipitate them, however, I like to know the underlying mechanisms in which phages become precipitated. The mechanisms of PEG and its final...

21 February 2017 8,996 4 View

PCANCTAB 5C phagemic copy number ?

hello every body I am using one of pCANTAB vectors (pCANTAB 5C) for my project. there is not details about this type of vector. how can find its details genetic map? since it originated from a pUC...

04 February 2016 4,563 1 View

What is the negative control for panning protocol?

Hi there, Im doing panning protocol with Nunc immunotube according to nature protocols. i coat one immunotube with 50 micro gram/ml of the peptide and one immunotube with no petide and all the...

07 September 2015 3,265 2 View

How can i disperse Gold nanopowder in polar or non-polar solvents?

Hi there I wanna conjugate a water miscible polymer to a 15 nm spherical gold nanopowder. since the nanogold is not soluble in water, can I dissolve the nanogold powder into non-polar solvents...

01 January 1970 1,730 6 View