Sevda Pouraghaei

5 Questions 6 Answers 0 Followers

Questions related from Sevda Pouraghaei

Any thoughts on the very low A260/230 ratios of isolated total RNA?

I have encapsulated my stem cells inside alginate hydrogel, I usualy use Thermofisher algimatrix dissolving buffer (https://www.thermofisher.com/order/catalog/product/A1134001) for isolating my...

08 August 2018 7,772 4 View

What happens when matrigel becomes gel?

I am trying to culture my stem cells in a 3D condition in the presence of Matrigel. Some times I am not fast enough and can't handle Matrigel properly; therefore, it becomes gel and doesn't mix...

11 November 2017 2,580 4 View

What is the best way of RNA extraction from tough embryoid bodies?

My embryoid bodies are very tough, it is very difficult to break down these cell aggregates and extract RNA just with pipetting. Does any one have a better idea?

10 October 2017 1,018 1 View

What is the cheapest media for culturing human embryonic stem cells?

What is the most basic culture media for cultivation of human embryonic stem cells?I mean how we can prepare a cheaper media to culture these cells?which ingredients can be omitted?

11 November 2016 732 5 View

What is the difference of human embryonic stem cells from mouse embryonic stem cells?

Hi dear friends, Would you please tell me is there any difference between culturing method and protocol of human embryonic and mouse embryonic stem cells?

10 October 2016 622 3 View