Why is the UV baseline drifting down during ion-exchange with FPLC?

Buffer A contains 25mM MES pH 6 10mM NaCl and 10mM BME buffer B contains 25mM MES pH 6 500mM NaCl and 10mM BME. When we run an increasing gradient of buffer B for ion exchange protein...

06 June 2019 7,336 4 View