Sergio Valbuena

3 Questions 4 Answers 0 Followers

Questions related from Sergio Valbuena

Fast-expressing fluorescent proteins for detecting TRAPed cells (fos-CreERT2) in mammalian neurons in vivo?

Good morning, I am trying to find a fluorescent protein with fast expression time (ideally hours or 1 day from expression induction to maturation). I want to use TRAP2 mice (Fos-CreERT2) and...

28 April 2020 5,831 0 View

Differences between theta burst stimulation (TBS) protocols

Dear colleages, I'm trying to evoke LTP in CA1 of 2-3 months old mice using a TBS protocol. Up to now, I've tried the two following ones: a.- Bursts of 4 pulses at 100 Hz, given 10 times and...

09 May 2017 9,175 1 View

Can anyone troubleshoot my procedure for immunohistochemistry in cultured hippocampal slices?

I'm starting to do hippocampal slice cultures of P5-P7 postnatal mice. I am interested in the establishment of sprouting at the mossy fibers, by culturing the slices with pilocarpine. I have...

06 May 2014 4,650 4 View