Sergio Gabriel Lopez

4 Questions 23 Answers 0 Followers

Questions related from Sergio Gabriel Lopez

Any good substitute for chromium trioxide when it comes to fixing microscopy samples?

Hello. I came across a fixation protocol for optical microscopy that employs chromium trioxide as a fixative. I was wondering if you guys could point me towards a less-toxic substitute for this...

09 January 2020 4,794 3 View

How likely is it for a fluorescent protein (e.g., GFP) to be quenched by the protein to which it is attached?

I have two proteins: (1) a FRET control which consists in protein X tagged with GFP only and (2) a protein of interest which consists in protein X tagged both with GFP (FRET donor) and mCherry...

28 November 2019 6,503 7 View

Can ultrasonication decompose DMSO?

We are implementing a protocol in wich DMSO must be sonicated for 15 h. After the sonication the DMSO has a very foul smell. Can this be related to thermal decomposition of the DMSO due to the...

18 December 2015 628 9 View

Why are magic angle conditions necessary when performing time-resolved fluorescence measurements on immobile samples?

I understand why magic angle conditions are necessary when performing steady-state fluorescence measurements on immobile samples (i.e., films), since I know that the transmission efficiency of the...

27 February 2015 1,631 5 View